Proteintech, 21348-1-AP, TMX4 Polyclonal antibody

Size: 20ul / 150ul The TMX4 (21348-1-AP) by Proteintech is a Polyclonal antibody targeting TMX4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 21348-1-AP targets TMX4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, A375 cells, human heart tissue, L02 cells Positive IHC detected in: human brain tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Background Information TMX4(Thioredoxin-related transmembrane protein 4) is also named as KIAA1162, TXNDC13. It is a type I transmembrane protein, with a Trx-like domain facing the ER lumen and may be to enable protein folding in cooperation with these proteins consisting of folding complex in the ER. It could work as a reductase in the environment of the ER(PMID: 20056998). The full length protein has a 23 amino acid signal peptide. This antibody is specific to TMX4. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15696 Product name: Recombinant human TMX4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 184-349 aa of BC033787 Sequence: PAWCSYVFFVIATLVFGLFMGLVLVVISECFYVPLPRHLSERSEQNRRSEEAHRAEQLQDAEEEKDDSNEEENKDSLVDDEEEKEDLGDEDEAEEEEEEDNLAAGVDEERSEANDQGPPREDGVTREEVEPEEAEEGISEQPCPADTEVVEDSLRQRKSQHADKGL Predict reactive species Full Name: thioredoxin-related transmembrane protein 4 Calculated Molecular Weight: 349 aa, 39 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC033787 Gene Symbol: TMX4 Gene ID (NCBI): 56255 RRID: AB_10733353 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H1E5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.