Proteintech, 21406-1-AP, TGM3 Polyclonal antibody

Size: 20ul / 150ul The TGM3 (21406-1-AP) by Proteintech is a Polyclonal antibody targeting TGM3 in WB, ELISA applications with reactivity to human samples 21406-1-AP targets TGM3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SMMC-7721 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information TGM3 (Transglutaminase 3) is a Protein Coding gene. Diseases associated with TGM3 include Uncombable Hair Syndrome 2 and Uncombable Hair Syndrome 1. Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. TG3 is expressed as an inactive 77 kDa zymogen which must be activated by limited proteolytic processing into two fragments (44 kDa and 30 kDa) of which the larger, N-terminal fragment carries the catalytic activity (PMID: 35328331). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16131 Product name: Recombinant human TGM3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 445-631 aa of BC109076 Sequence: GSDQERQVFQKALGKLKPNTPFAATSSMGLETEEQEPSIIGKLKVAGMLAVGKEVNLVLLLKNLSRDTKTVTVNMTAWTIIYNGTLVHEVWKDSATMSLDPEEEAEHPIKISYAQYEKYLKSDNMIRITAVCKVPDESEVVVERDIILDNPTLTLEVLNEARVRKPVNVQMLFSNPLDEPVRDCVLM Predict reactive species Full Name: transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase) Calculated Molecular Weight: 693 aa, 77 kDa Observed Molecular Weight: 77 kDa; 30-35 kDa; 44-50 kDa GenBank Accession Number: BC109076 Gene Symbol: TGM3 Gene ID (NCBI): 7053 RRID: AB_3085655 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q08188 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.