Proteintech, 21429-1-AP, TBCCD1 Polyclonal antibody

Size: 20ul / 150ul The TBCCD1 (21429-1-AP) by Proteintech is a Polyclonal antibody targeting TBCCD1 in WB, IF/ICC, ELISA applications with reactivity to human samples 21429-1-AP targets TBCCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, human testis tissue Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TBCC-domain containing 1 (TBCCD1) belongs to the tubulin-binding cofactor C (TBCC) family, which is a new human pericentriolar matrix component (PMID: 24101722). TBCCD1 localizes at the centrosome and at the spindle midzone, midbody and basal bodies of primary and motile cilia. Knockdown of TBCCD1 in RPE-1 cells caused the dissociation of the centrosome from the nucleus and disorganization of the Golgi apparatus. TBCCD1 is a key regulator of centrosome positioning and consequently of internal cell organization (PMID: 20168327). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16032 Product name: Recombinant human TBCCD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 208-557 aa of BC025748 Sequence: AVVALSFLIEGTISRARKIYPLHELALWQPLHADSGFSKISKTFSFYKLETWLRSCLTGNPFGTSACLKSGKKLAWAHQVEGTTKRAKIACNTHVAPRMHRLVVMSQVYKQTLAKSSDTLAGAHVKIHRCNESFIYLLSPLRSVTIEKCRNSIFVLGPVGTTLHLHSCDNVKVIAVCHRLSISSTTGCIFHVLTPTRPLILSGNQTVTFAPFHTHYPMLEDHMARTGLATVPNYWDNPMVVCRENSDTRVFQLLPPCEFYVFIIPFEMEGDTTEIPGGLPSVYQKALGQREQKIQIWQKTVKEAHLTKDQRKQFQVLVENKFYEWLINTGHRQQLDSLVPPAAGSKQAAG Predict reactive species Full Name: TBCC domain containing 1 Calculated Molecular Weight: 557 aa, 64 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC025748 Gene Symbol: TBCCD1 Gene ID (NCBI): 55171 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q9NVR7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.