Proteintech, 21465-1-AP, IGSF21 Polyclonal antibody

Size: 20ul / 150ul The IGSF21 (21465-1-AP) by Proteintech is a Polyclonal antibody targeting IGSF21 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 21465-1-AP targets IGSF21 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue Positive IHC detected in: rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information IGSF21 (Immunoglobulin superfamily member 21) is a GPI-anchored protein that contains two immunoglobulin-like domains (Ig-like 1 and Ig-like 2). The Ig-like 1 domain of IGSF21 is indispensable for synaptogenic activity whereas its Ig-like 2 domain is secondarily responsible for the activity. It has been reported that IGSF21 selectively regulates inhibitory presynaptic differentiation through interacting with presynaptic neurexin2α and plays a crucial role in synaptic inhibition in the brain. (PMID: 28864826) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15062 Product name: Recombinant human IGSF21 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 117-467 aa of BC007618 Sequence: HVGIYDRATREKVVLASGNIFLNVMAPPTSIEVVAADTPAPFSRYQAQNFTLVCIVSGGKPAPMVYFKRDGEPIDAVPLSEPPAASSGPLQDSRPFRSLLHRDLDDTKMQKSLSLLDAENRGGRPYTERPSRGLTPDPNILLQPTTENIPETVVSREFPRWVHSAEPTYFLRHSRTPSSDGTVEVRALLTWTLNPQIDNEALFSCEVKHPALSMPMQAEVTLVAPKGPKIVMTPSRARVGDTVRILVHGFQNEVFPEPMFTWTRVGSRLLDGSAEFDGKELVLERVPAELNGSMYRCTAQNPLGSTDTHTRLIVFENPNIPRGTEDSNGSIGPTGARLTLVLALTVILELT Predict reactive species Full Name: immunoglobin superfamily, member 21 Calculated Molecular Weight: 467 aa, 52 kDa Observed Molecular Weight: 48-50 kDa GenBank Accession Number: BC007618 Gene Symbol: IGSF21 Gene ID (NCBI): 84966 RRID: AB_10734429 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96ID5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.