Proteintech, 21529-1-AP, FAM120A Polyclonal antibody

Size: 20ul / 150ul The FAM120A (21529-1-AP) by Proteintech is a Polyclonal antibody targeting FAM120A in IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 21529-1-AP targets FAM120A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information FAM120A (also named OSSA/C9ORF10) is mapped to chromosome 9q22.31, which was first reported to be a component of Purα-containing mRNP complexes. FAM120A as a transcription co-activator that couples transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1-serine/arginine-rich protein kinase 2 (SRPK2) signaling. FAM120A is abundantly expressed in the cytoplasm of the GC cells and elevated FAM120A is observed in the scirrhous-type GC tissues compared with normal gastric mucosa. (PMID: 38565940, PMID: 37595559) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16149 Product name: Recombinant human FAM120A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 285-538 aa of BC111736 Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR Predict reactive species Full Name: family with sequence similarity 120A Calculated Molecular Weight: 1118 aa, 122 kDa Observed Molecular Weight: 125 kDa GenBank Accession Number: BC111736 Gene Symbol: FAM120A Gene ID (NCBI): 23196 RRID: AB_10860261 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NZB2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.