Proteintech, 21652-1-AP, Cardiac Troponin I Polyclonal antibody

Size: 20ul / 150ul The Cardiac Troponin I (21652-1-AP) by Proteintech is a Polyclonal antibody targeting Cardiac Troponin I in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 21652-1-AP targets Cardiac Troponin I in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse heart tissue, rat heart tissue, HEK-293 cells Positive IP detected in: mouse heart tissue Positive IHC detected in: mouse heart tissue, human heart tissue, mouse lung tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:20000-1:100000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:300-1:1200 Background Information Troponin I (TnI) is the inhibitory subunit of troponin; the thin filament regulatory complex that confers calcium sensitivity to striated muscle actomyosin ATPase activity. Cardiac Troponin I (cTnI) is encoded by theTNNI3gene located on chromosome 19 in humans.What is the molecular weight of cTnI?The molecular weight of cTnI is 24 kDa.What is the tissue specificity of cTnI?cTnI is produced exclusively by cardiac muscle.What are the post-translational modifications of cTnI?cTnI at Ser-23 and Ser-24 under adrenergic stimulation enhances the relaxation of cardiac muscle.What is the role of cTnI in disease?cTnI is considered to be one of the most specific and sensitive markers of myocardial damage such as acute myocardial infarction (AMI), as detection of cTnI in serum is widely accepted as a diagnostic tool (PMID: 15452154). Defects inTNNI3lead to cardiomyopathy familial hypertrophic type 7 (CMH7); an inherited heart disorder characterized by ventricular hypertrophy (PMID: 8205619). Defects inTNNI3can also cause cardiomyopathy familial restrictive type I (RCM1) (PMID: 20617149) and cardiomyopathy dilated type 2A (CMD2A) (PMID: 12531876). These disorders are characterized by the impaired filling of ventricles or ventricular dilation respectively. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16365 Product name: Recombinant human TNNI3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-110 aa of BC096165 Sequence: MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEE Predict reactive species Full Name: troponin I type 3 (cardiac) Calculated Molecular Weight: 210 aa, 24 kDa Observed Molecular Weight: 24-26 kDa GenBank Accession Number: BC096165 Gene Symbol: Cardiac Troponin I Gene ID (NCBI): 7137 RRID: AB_2878898 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P19429 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.