Proteintech, 21686-1-AP, Neurofibromin 2 Polyclonal antibody

Size: 20ul / 150ul The Neurofibromin 2 (21686-1-AP) by Proteintech is a Polyclonal antibody targeting Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 21686-1-AP targets Neurofibromin 2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, HEK-293 cells, Jurkat cells, SH-SY5Y cells, NIH/3T3 cells, MDCK cells, mouse brain tissue, rat brain tissue Positive IP detected in: HeLa cells Positive IHC detected in: mouse brain tissue, human meningioma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:16000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:750-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors. Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16265 Product name: Recombinant human NF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 245-595 aa of BC020257 Sequence: DPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFNLIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHNENSDRGGSSKHNTIKKLTLQSAKSRVAFFEEL Predict reactive species Full Name: neurofibromin 2 (merlin) Calculated Molecular Weight: 595 aa, 70 kDa Observed Molecular Weight: 66-70 kDa GenBank Accession Number: BC020257 Gene Symbol: NF2 Gene ID (NCBI): 4771 RRID: AB_10792410 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35240 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.