Proteintech, 21705-1-AP, UQCRC1 Polyclonal antibody

Size: 20ul / 150ul The UQCRC1 (21705-1-AP) by Proteintech is a Polyclonal antibody targeting UQCRC1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 21705-1-AP targets UQCRC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, human brain tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, human heart tissue, mouse large intestine tissue, mouse kidney tissue, mouse spleen tissue, L02 cells, mouse liver tissue, rat liver tissue, Jurkat cells Positive IP detected in: HeLa cells Positive IHC detected in: human normal colon, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:100-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16322 Product name: Recombinant human UQCRC1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 159-480 aa of BC009586 Sequence: SQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF Predict reactive species Full Name: ubiquinol-cytochrome c reductase core protein I Calculated Molecular Weight: 480 aa, 53 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC009586 Gene Symbol: UQCRC1 Gene ID (NCBI): 7384 RRID: AB_10734437 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31930 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.