Proteintech, 21770-1-AP, PNMA5 Polyclonal antibody

Size: 20ul / 150ul The PNMA5 (21770-1-AP) by Proteintech is a Polyclonal antibody targeting PNMA5 in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse samples 21770-1-AP targets PNMA5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HT-1376 cells, TT cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse testis tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information PNMA5 is a member of the paraneoplastic Ma antigen (PNMA) family proteins which share relatively conserved protein sequences and are involved in the regulation of growth, apoptosis, or fate determination in different cell types (PMID: 29378289). PNMA5 appears to be involved in the association areas of the neocortex during primate evolution (PMID: 19366867). PNMA5 has been reported to play roles in the pathological development and metastasis of some cancers, including colorectal cancer, ovarian cancer, and lung cancer (PMID: 35559417; 35117731; 34136487). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16414 Product name: Recombinant human PNMA5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 133-448 aa of BC101112 Sequence: SLDAEVMPQVRSPPLEPPKESMWYRKLKVFSGTASPSPGEETFEDWLEQVTEIMPIWQVSEVEKRRRLLESLRGPALSIMRVLQANNDSITVEQCLDALKQIFGDKEDFGASQFRFLQTSPKIGEKVSTFLLRLEPLLQKAVHKSPLSVRSTDMIRLKHLLARVAMTPALRGKLELLDQRGCPPNFLELMKLIRDEEEWENTEAVMKNKEKPSGRGRGASGRQARAEASVSAPQATVQARSFSDSSPQTIQGGLPPLVKRRRLLGSESTRGEDHGQATYPKAENQTPGREGPQAAGEELGNEAGAGAMSHPKPWET Predict reactive species Full Name: paraneoplastic antigen like 5 Calculated Molecular Weight: 448 aa, 50 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC101112 Gene Symbol: PNMA5 Gene ID (NCBI): 114824 RRID: AB_3669374 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96PV4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.