Proteintech, 21852-1-AP, SOAT2 Polyclonal antibody

Size: 20ul / 150ul The SOAT2 (21852-1-AP) by Proteintech is a Polyclonal antibody targeting SOAT2 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 21852-1-AP targets SOAT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, L02 cells Positive IHC detected in: human liver tissue, human small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information SOAT2, also named as ACAT2, belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. SOAT2 is expressed preferentially in enterocytes of the intestine and hepatocytes of the liver. The role of SOAT2 in generating esterified cholesterol (EC) that is incorporated directly into atherogenic lipoproteins has made it an attractive target in dyslipidemia management and prevention. (PMID: 29246491) Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16364 Product name: Recombinant human SOAT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-119 aa of BC096090 Sequence: MEPGGARLRLQRTGGLGGERERQPCGDGNTETHRAPDLVQWTRHMEAVKAQLLEQAQGQLRELLDRAMREAIQSYPSQDKPLPPPPPGSLSRTQEPSLGKQKVFIIRKSLLDELMEVQH Predict reactive species Full Name: sterol O-acyltransferase 2 Calculated Molecular Weight: 522 aa, 60 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC096090 Gene Symbol: SOAT2 Gene ID (NCBI): 8435 RRID: AB_11183043 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O75908 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.