Proteintech, 21856-1-AP, KIDINS220 Polyclonal antibody

Size: 20ul / 150ul The KIDINS220 (21856-1-AP) by Proteintech is a Polyclonal antibody targeting KIDINS220 in WB, IHC, IP, ELISA applications with reactivity to human samples 21856-1-AP targets KIDINS220 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, human brain tissue, Raji cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human ovarian cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa. Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16448 Product name: Recombinant human KIDINS220 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1374-1672 aa of BC130610 Sequence: LDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL Predict reactive species Full Name: kinase D-interacting substrate, 220kDa Calculated Molecular Weight: 1672 aa, 187 kDa Observed Molecular Weight: 186-197 kDa GenBank Accession Number: BC130610 Gene Symbol: KIDINS220 Gene ID (NCBI): 57498 RRID: AB_10836934 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9ULH0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.