Proteintech, 21861-1-AP, DOCK4 Polyclonal antibody

Size: 20ul / 150ul The DOCK4 (21861-1-AP) by Proteintech is a Polyclonal antibody targeting DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 21861-1-AP targets DOCK4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HEK-293T cells Positive IP detected in: HeLa cells, HEK-293T cells Positive IHC detected in: human ovary tumor tissue, human breast cancer tissue, human prostate cancer tissue, human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:150-1:600 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information DOCK4, originally identified as a product of a gene which is deleted during tumor progression, is a member of DOCK180 family proteins. Dock4 has been found recently to be associated with several neuropsychiatric diseases, including autism, dyslexia, and schizophrenia. Multiple studies in fibroblasts then confirmed that Dock4 is capable of controlling cell migration by transducing several upstream signals, such as Wnt, platelet-derived growth factor, and RhoG, toward activation of Rac1. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16516 Product name: Recombinant human DOCK4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1703-2011 aa of BC117689 Sequence: TGQSESSDEVFNMQPSPSTSSLSSTHSASPNVTSSAPSSARASPLLSDKHKHSRENSCLSPRERPCSAIYPTPVEPSQRMLFNHIGDGALPRSDPNLSAPEKAVNPTPSSWSLDSGKEAKNMSDSGKLISPPVPPRPTQTASPARHTTSVSPSPAGRSPLKGSVQSFTPSPVEYHSPGLISNSPVLSGSYSSGISSLSRCSTSETSGFENQVNEQSAPLPVPVPVPVPSYGGEEPVRKESKTPPPYSVYERTLRRPIPLPHSLSIPVTSEPPALPPKPLAARSSHLENGARRTDPGPRPRPLPRKVSQL Predict reactive species Full Name: dedicator of cytokinesis 4 Calculated Molecular Weight: 2011 aa, 230 kDa Observed Molecular Weight: 225 kDa GenBank Accession Number: BC117689 Gene Symbol: DOCK4 Gene ID (NCBI): 9732 RRID: AB_2878930 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N1I0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.