Proteintech, 21870-1-AP, DUS4L Polyclonal antibody

Size: 20ul / 150ul The DUS4L (21870-1-AP) by Proteintech is a Polyclonal antibody targeting DUS4L in WB, IHC, ELISA applications with reactivity to human, mouse samples 21870-1-AP targets DUS4L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information tRNA-dihydrouridine synthase 4-like belongs to the dus family.It catalyzes the synthesis of dihydrouridine and a modified base found in the D-loop of most tRNAs.Researches showed that DUS4L was alsophosphorylated in vitro by a Ca2+ calmodulin-activated kinase. It has 2 isoforms produced by alternative splicing .One, at 35 kDa, comigrated with a second cAMP-stimulated RIS-ROS phosphoprotein,DUS4L,which was also detected in the cytoskeleton.This antibody recognize the C-terminal of DUS4L. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16145 Product name: Recombinant human DUS4L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-197 aa of BC111774 Sequence: AMAEGYGACLINKPELVQDMVKQVRNQVETPGFSVSIKIRIHDDLKRTVDLCQKAEATGVSWITVHGRTAEERHQPVHYDSIKIIKENMSIPVIANGDIRSLKEAENVWRITGTDGVMVARGLLANPAMFAGYEETPLKCIWDWVDIALELGTPYMCFHQHLMYMMEKITSRQEKRVFNALSSTSAIIDYLTDHYGI Predict reactive species Full Name: dihydrouridine synthase 4-like (S. cerevisiae) Calculated Molecular Weight: 317 aa, 36 kDa Observed Molecular Weight: 22 kDa, 30 kDa GenBank Accession Number: BC111774 Gene Symbol: DUS4L Gene ID (NCBI): 11062 RRID: AB_2878933 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95620 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.