Proteintech, 21913-1-AP, TMEM65 Polyclonal antibody

Size: 20ul / 150ul The TMEM65 (21913-1-AP) by Proteintech is a Polyclonal antibody targeting TMEM65 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 21913-1-AP targets TMEM65 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Neuro-2a cells, mouse brain tissue, rat brain tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Huh-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information TMEM65 (Transmembrane protein 65) is an inner-mitochondrial-membrane protein essential for calcium homeostasis and organelle integrity. By binding to and stabilizing the Na⁺/Ca²⁺ exchanger NCLX, it facilitates mitochondrial Ca²⁺ efflux, thereby regulating oxidative phosphorylation and limiting ROS production. In cardiomyocytes, TMEM65 localizes to intercalated discs where it interacts with connexin-43, ensuring normal electrical conduction and preventing arrhythmia. Loss-of-function mutations cause mitochondrial myopathy with neurodevelopmental delay, whereas its over-expression in gastric cancer activates the YWHAZ-PI3K-Akt-mTOR axis, promoting tumorigenesis. Thus, TMEM65 links mitochondrial quality control to cytoplasmic signaling, positioning it as a potential therapeutic target in metabolic and oncologic diseases. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag15509 Product name: Recombinant human TMEM65 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-178 aa of BC032396 Sequence: MEALNTAQGARDFIYSLHSTERSCLLKELHRFESIAIAQEKLEAPPPTPGQLRYVFIHNAIPFIGFGFLDNAIMIVAGTHIEMSIGIILGISTMAAAALGNLVSDLAGLGLAGYVEALASRLGLSIPDLTPKQVDMWQTRLSTHLGKAVGVTIGCILGMFPLIFFGGGEEDEKLETKS Predict reactive species Full Name: transmembrane protein 65 Calculated Molecular Weight: 240 aa, 26 kDa Observed Molecular Weight: 20-25 kDa GenBank Accession Number: BC032396 Gene Symbol: TMEM65 Gene ID (NCBI): 157378 RRID: AB_2918075 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6PI78 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.