Proteintech, 22047-1-AP, WIPF1 Polyclonal antibody

Size: 20ul / 150ul The WIPF1 (22047-1-AP) by Proteintech is a Polyclonal antibody targeting WIPF1 in WB, IHC, ELISA applications with reactivity to human samples 22047-1-AP targets WIPF1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information WASP interacting protein family member 1(WIPF1), also known as WIP, is a ubiquitously expressed proline-rich multidomain protein and plays a role in the reorganization of the actin cytoskeleton. Mutations in the WIPF1 binding site of the Wiskott-Aldrich syndrome protein or in WIPF1 itself cause Wiskott-Aldrich syndrome, which predisposes people to cancer, such as leukemia and lymphoma(PMID: 27863429). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16975 Product name: Recombinant human WIPF1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 14-238 aa of BC110288 Sequence: TFALANTEKPTLNKTEQAGRNALLSDISKGKKLKKTVTNDRSAPILDKPKGAGAGGGGGGFGGGGGFGGGGGGGGGGSFGGGGPPGLGGLFQAGMPKLRSTANRDNDSGGSRPPLLPPGGRSTSAKPFSPPSGPGRFPVPSPGHRSGPPEPQRNRMPPPRPDVGSKPDSIPPPVPSTPRPIQSSLHNRGSPPVPGGPRQPSPGPTPPPFPGNRGTALGGGSIRQS Predict reactive species Full Name: WAS/WASL interacting protein family, member 1 Calculated Molecular Weight: 403aa,40 kDa; 503aa,51 kDa Observed Molecular Weight: 51 kDa GenBank Accession Number: BC110288 Gene Symbol: WIPF1 Gene ID (NCBI): 7456 RRID: AB_3085682 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O43516 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.