Proteintech, 22053-1-AP, MAGED1 Polyclonal antibody

Size: 20ul / 150ul The MAGED1 (22053-1-AP) by Proteintech is a Polyclonal antibody targeting MAGED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 22053-1-AP targets MAGED1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, mouse placenta tissue Positive IP detected in: A549 cells Positive IHC detected in: mouse placenta tissue, human skin tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information MAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of protein. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527). Three transcript variants encoding two different isoforms (86kDa and 92 kDa) have been found for the gene of MAGED1. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17047 Product name: Recombinant human MAGED1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC014070 Sequence: MAQKMDCGAGLLGFQNPDACRAVCHPLPQPPASTLPLSAFPTLCDPPYSQLRDPPAVLSCYCTPLGASPAPAEASVEDSALLMQTLMEAIQISEAPPTNQATAAASPQSSQPPTANEMADIQVSAAAARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTKVGPNATYNFSQSLNANDLANSRPKTPFKAWNDTTKAPTADTQTQNVNQAKMATSQADIETDPGISEPDGATAQTSADGSQAQNLESRTIIRGKRTRKINNLNVEENSSGDQRRAPLAAGTWRSAPVPVTTQNPPGA Predict reactive species Full Name: melanoma antigen family D, 1 Calculated Molecular Weight: 834 aa, 92 kDa Observed Molecular Weight: 95-100 kDa GenBank Accession Number: BC014070 Gene Symbol: MAGED1 Gene ID (NCBI): 9500 RRID: AB_11182601 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y5V3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.