Proteintech, 22063-1-AP, PDE6B Polyclonal antibody

Size: 20ul / 150ul The PDE6B (22063-1-AP) by Proteintech is a Polyclonal antibody targeting PDE6B in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples 22063-1-AP targets PDE6B in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse eye tissue, A549 cells, rat eye tissue Positive IP detected in: Y79 cells Positive IHC detected in: rat eye tissue, mouse eye tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat eye tissue, mouse eye tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits (PDE6A and PDE6B) and it is expressed in human lungs (PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation (PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) (PMID:8075643). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17139 Product name: Recombinant human PDE6B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-88 aa of BC000249 Sequence: MSLSEEQARSFLDQNPDFARQYFGKKLSPENVAAACEDGCPPDCDSLRDLCQVEESTALLELVQDMQESINMERVVFKVLRRLCTLLQ Predict reactive species Full Name: phosphodiesterase 6B, cGMP-specific, rod, beta Calculated Molecular Weight: 854 aa, 98 kDa Observed Molecular Weight: 98 kDa GenBank Accession Number: BC000249 Gene Symbol: PDE6B Gene ID (NCBI): 5158 RRID: AB_2619668 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35913 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.