Proteintech, 22124-1-AP, TBC1D1 Polyclonal antibody

Size: 20ul / 150ul The TBC1D1 (22124-1-AP) by Proteintech is a Polyclonal antibody targeting TBC1D1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse samples 22124-1-AP targets TBC1D1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: LNCaP cells, HeLa cells, HEK-293T cells, Jurkat cells, MCF-7 cells Positive IP detected in: Jurkat cells Positive IHC detected in: mouse kidney tissue, human testis tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag5406 Product name: Recombinant human TBC1D1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 813-1168 aa of BC050321 Sequence: LAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD Predict reactive species Full Name: TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 Calculated Molecular Weight: 133 kDa Observed Molecular Weight: 150-165 kDa GenBank Accession Number: BC050321 Gene Symbol: TBC1D1 Gene ID (NCBI): 23216 RRID: AB_2827874 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86TI0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.