Proteintech, 22138-1-AP, RYK Polyclonal antibody

Size: 20ul / 150ul The RYK (22138-1-AP) by Proteintech is a Polyclonal antibody targeting RYK in WB, ELISA applications with reactivity to human samples 22138-1-AP targets RYK in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: COLO 320 cells, A549 cells, Caco-2 cells, Jurkat cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Tyrosine-protein kinase RYK may be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A, and WNT5A. It is involved in neuron differentiation, axon guidance, corpus callosum establishment, and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region, allowing the C-terminal intracellular product to translocate from the cytoplasm to the nucleus, which plays a crucial role in neuronal development. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17540 Product name: Recombinant human RYK protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 261-610 aa of BC153090 Sequence: DDSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSSLGYPTLRIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILIDEKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCIEEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIACGMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDNENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEMAAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHAALGAYV Predict reactive species Full Name: RYK receptor-like tyrosine kinase Calculated Molecular Weight: 610 aa, 68 kDa Observed Molecular Weight: 68 kDa GenBank Accession Number: BC153090 Gene Symbol: RYK Gene ID (NCBI): 6259 RRID: AB_11232602 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P34925 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.