Proteintech, 22184-1-AP, Connexin 45 Polyclonal antibody

Size: 20ul / 150ul The Connexin 45 (22184-1-AP) by Proteintech is a Polyclonal antibody targeting Connexin 45 in WB, IHC, ELISA applications with reactivity to human, mouse samples 22184-1-AP targets Connexin 45 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse heart tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106). Specification Tested Reactivity: human, mouse Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag16742 Product name: Recombinant human GJC1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 248-396 aa of BC096213 Sequence: WEMLHLGFGTIRDSLNSKRRELEDPGAYNYPFTWNTPSAPPGYNIAVKPDQIQYTELSNAKIAYKQNKANTAQEQQYGSHEENLPADLEALQREIRMAQERLDLAVQAYSHQNNPHGPREKKAKVGSKAGSNKSTASSKSGDGKNSVWI Predict reactive species Full Name: gap junction protein, gamma 1, 45kDa Calculated Molecular Weight: 396 aa, 45 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC096213 Gene Symbol: Connexin-45 Gene ID (NCBI): 10052 RRID: AB_3085686 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P36383 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.