Proteintech, 22212-1-AP, TMF1 Polyclonal antibody

Size: 20ul / 150ul The TMF1 (22212-1-AP) by Proteintech is a Polyclonal antibody targeting TMF1 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 22212-1-AP targets TMF1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, Jurkat cells, mouse colon tissue, rat brain tissue, human brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: human colon cancer tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:150-1:600 Background Information TMF1, also named as TMF and ARA160, is a potential coactivator of the androgen receptor. It mediates STAT3 degradation. The MW of TMF1 is about 160 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag17537 Product name: Recombinant human TMF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 783-1093 aa of BC126123 Sequence: GSQTSSWEKLEKNLSDRLGESQTLLAAAVERERAATEELLANKIQMSSMESQNSLLRQENSRFQAQLESEKNRLCKLEDENNRYQVELENLKDEYVRTLEETRKEKTLLNSQLEMERMKVEQERKKAIFTQETIKEKERKPFSVSSTPTMSRSSSISGVDMAGLQTSFLSQDESHDHSFGPMPISANGSNLYDAVRMGAGSSIIENLQSQLKLREGEITHLQLEIGNLEKTRSIMAEELVKLTNQNDELEEKVKEIPKLRTQLRDLDQRYNTILQMYGEKAEEAEELRLDLEDVKNMYKTQIDELLRQSLS Predict reactive species Full Name: TATA element modulatory factor 1 Calculated Molecular Weight: 1093 aa, 123 kDa Observed Molecular Weight: 150-160 kDa GenBank Accession Number: BC126123 Gene Symbol: TMF1 Gene ID (NCBI): 7110 RRID: AB_2879031 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P82094 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.