Proteintech, 22282-1-AP, MYH1-motor domain Polyclonal antibody

Size: 20ul / 150ul The MYH1-motor domain (22282-1-AP) by Proteintech is a Polyclonal antibody targeting MYH1-motor domain in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse samples 22282-1-AP targets MYH1-motor domain in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human skeletal muscle tissue Positive IHC detected in: human skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information MYH1 (MyHC-2x) encodes the IIX isoform of myosin heavy chain (MyHC). Myosin is a large, ubiquitous, motor protein that generates force through its interaction with actin, thus involving it in a number of cellular processes including cytokinesis, karyokinesis, cell migration, and muscle contraction. Muscle fibers can be divided as type 1 (slow) and type 2 (fast). MYH1 belongs to type 2. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, heavy chain 1, skeletal muscle, adult Calculated Molecular Weight: 223 kDa Observed Molecular Weight: 223 kDa GenBank Accession Number: NM_005963 Gene Symbol: MYH1 Gene ID (NCBI): 4619 RRID: AB_2879059 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P12882 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.