Proteintech, 22287-1-AP, MYH3 Polyclonal antibody

Size: 20ul / 150ul The MYH3 (22287-1-AP) by Proteintech is a Polyclonal antibody targeting MYH3 in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 22287-1-AP targets MYH3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse embryo tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: rat heart tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: myosin, heavy chain 3, skeletal muscle, embryonic Calculated Molecular Weight: 234 kDa Observed Molecular Weight: 220-230 kDa GenBank Accession Number: NM_002470 Gene Symbol: MYH3 Gene ID (NCBI): 4621 RRID: AB_2879060 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P11055 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.