Proteintech, 22519-1-AP, SRGAP2 Polyclonal antibody

Size: 20ul / 150ul The SRGAP2 (22519-1-AP) by Proteintech is a Polyclonal antibody targeting SRGAP2 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 22519-1-AP targets SRGAP2 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, HEK-293 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse brain tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information SLIT-ROBO Rho GTPase-activating protein 2 (SRGAP2) is also named as ARHGAP34, FNBP2, KIAA0456 and SRGAP2A. SRGAP2 plays important roles in axon guidance, neuronal migration, synapse formation, and nerve regeneration. SRGAP2 protein was first expressed in the retina of normal mice at the embryonic stage and was mainly located in the mature retinal ganglion cell layer and the inner nuclear layer (PMID: 37056152). SRGAP2 plays a key role in neuronal morphogenesis and migration mainly during development of the cerebral cortex (PMID: 20810653, PMID: 27373832, PMID: 28333212). SRGAP2 are critical for neuronal migration through inactivation of Rho GTPases Cdc42, Rac1, and RhoA (PMID: 20810653). SRGAP2A promotes synaptic maturation, and ultimately the synaptic accumulation of AMPA and GABAA receptors, by interacting with key components of both excitatory and inhibitory postsynaptic scaffolds, Homer and Gephyrin (PMID: 27373832). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18278 Product name: Recombinant human SRGAP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 784-944 aa of BC132874 Sequence: VQDTEDGVVERSSPKSEIEVISEPPEEKVTARAGASCPSGGHVADIYLANINKQRKRPESGSIRKTFRSDSHGLSSSLTDSSSPGVGASCRPSSQPIMSQSLPKEGPDKCSISGHGSLNSISRHSSLKNRLDSPQIRKTATAGRSKSFNNHRPMDPEVIAQ Predict reactive species Full Name: SLIT-ROBO Rho GTPase activating protein 2 Calculated Molecular Weight: 1071 aa, 121 kDa Observed Molecular Weight: 121 kDa GenBank Accession Number: BC132874 Gene Symbol: SRGAP2 Gene ID (NCBI): 23380 RRID: AB_2879114 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: O75044 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.