Proteintech, 22585-1-AP, BCR Polyclonal antibody

Size: 20ul / 150ul The BCR (22585-1-AP) by Proteintech is a Polyclonal antibody targeting BCR in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, monkey samples 22585-1-AP targets BCR in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, monkey samples. Tested Applications Positive WB detected in: Y79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia. Specification Tested Reactivity: human, monkey Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18281 Product name: Recombinant human BCR protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-350 aa of BC066122 Sequence: MVDPVGFAEAWKAQFPDSEPPRMELRSVGDIEQELERCKASIRRLEQEVNQERFRMIYLQTLLAKEKKSYDRQRWGFRRAAQAPDGASEPRASASRPQPAPADGADPPPAEEPEARPDGEGSPGKARPGTARRPGAAASGERDDRGPPASVAALRSNFERIRKGHGQPGADAEKPFYVNVEFHHERGLVKVNDKEVSDRISSLGSQAMQMERKKSQHGAGSSVGDASRPPYRGRSSESSCGVDGDYEDAELNPRFLKDNLIDANGGSRPPWPPLEYQPYQSIYVGGMMEGEGKGPLLRSQSTSEQEKRLTWPRRSYSPRSFEDCGGGYTPDCSSNENLTSSEEDFSSGQS Predict reactive species Full Name: breakpoint cluster region Calculated Molecular Weight: 143 kDa Observed Molecular Weight: 150-160 kDa GenBank Accession Number: BC066122 Gene Symbol: BCR Gene ID (NCBI): 613 RRID: AB_2879127 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: P11274 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.