Proteintech, 22623-1-AP, FKBP52 Polyclonal antibody

Size: 20ul / 150ul The FKBP52 (22623-1-AP) by Proteintech is a Polyclonal antibody targeting FKBP52 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 22623-1-AP targets FKBP52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells Positive IHC detected in: human colon tissue, human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: PC-3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18704 Product name: Recombinant human FKBP52 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-286 aa of BC007924 Sequence: MTAEEMKATESGAQSAPLPMEGVDISPKQDEGVLKVIKREGTGTEMPMIGDRVFVHYTGWLLDGTKFDSSLDRKDKFSFDLGKGEVIKAWDIAIATMKVGEVCHITCKPEYAYGSAGSPPKIPPNATLVFEVELFEFKGEDLTEEEDGGIIRRIQTRGEGYAKPNEGAIVEVALEGYYKDKLFDQRELRFEIGEGENLDLPYGLERAIQRMEKGEHSIVYLKPSYAFGSVGKEKFQIPPNAELKYELHLKSFEKAKESWEMNSEEKLEQSTIVKERGTVYFKEGKY Predict reactive species Full Name: FK506 binding protein 4, 59kDa Calculated Molecular Weight: 52 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC007924 Gene Symbol: FKBP52 Gene ID (NCBI): 2288 RRID: AB_2879136 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q02790 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.