Proteintech, 22676-1-AP, PPP4R2 Polyclonal antibody

Size: 20ul / 150ul The PPP4R2 (22676-1-AP) by Proteintech is a Polyclonal antibody targeting PPP4R2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 22676-1-AP targets PPP4R2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells Positive IP detected in: HepG2 cells Positive IHC detected in: human liver cancer tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information PPP4R2 is a regulatory subunit of protein phosphatase-4 (PPP4), a protein serine/threonine phosphatase implicated in microtubule organization at centrosomes and DNA double-strand break repair. The deduced 453-amino acid protein contains several potential phosphorylation sites and has a calculated molecular mass of 50.4 kD. Immunofluorescent analysis of HeLa and A431 cells revealed prominent localization of PPP4R2 at centrosomes, with weaker staining of cytoplasm and nucleus(PMID: 10769191). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18486 Product name: Recombinant human PPP4R2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-205 aa of BC110889 Sequence: MDVERLQEALKDFEKRGKKEVCPVLDQFLCHVAKTGETIIPFTIQRLCELLTDPRRNYTGTDKFLRGVEKNVMVVSCVYPSSEKNNSNSLNRMNGVMFPGNSPSYTERSNINGPGTPRPLNRPKVSLSAPMTTNGLPESTDSKEANLQQNEEKNHSDSSTSESEVSSVSPLKNKHPDEDAVEAEGHEVKRLRFDKEGEVRETASQ Predict reactive species Full Name: protein phosphatase 4, regulatory subunit 2 Calculated Molecular Weight: 360aa,40 kDa; 495aa,55 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC110889 Gene Symbol: PPP4R2 Gene ID (NCBI): 151987 RRID: AB_2879149 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NY27 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.