Proteintech, 22809-1-AP, Dectin-1 Polyclonal antibody

Size: 20ul / 150ul The Dectin-1 (22809-1-AP) by Proteintech is a Polyclonal antibody targeting Dectin-1 in WB, ELISA applications with reactivity to human samples 22809-1-AP targets Dectin-1 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HL-60 cells, THP-1 cells, U-937 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information Dectin-1, also known as CLEC7A or CD369, is a type II membrane receptor consisting of an extracellular C-terminal C-type lectin domain, a short stalk region, a single transmembrane domain and a short N-terminal cytoplasmic tail, which contains an immunoreceptor tyrosine-based activation motif (PMID: 10779524; 21612412). Dectin-1 is expressed on monocytes, macrophages, neutrophils, dendritic cells and a subpopulation of T cells (PMID: 12244185). Dectin-1 plays a role in the innate immune response. It functions as a pattern-recognition receptor and recognizes a variety of β-glucans from fungi, plants and bacteria. Dectin-1 may also participate in orchestrating the adaptive immune response (PMID: 21612412). The apparent molecular weight is larger than the calculated molecular weight due to glycosylation (PMID: 10779524). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18924 Product name: Recombinant human CLEC7A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-77 aa of BC013385 Sequence: MEYHPDLENLDEDGYTQLHFDSQSNTRIAVVSEKGSCAASPPWRLIAVILGILCLVILVIAVVLGTMAGFKAVEFKG Predict reactive species Full Name: C-type lectin domain family 7, member A Calculated Molecular Weight: 247 aa, 28 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC013385 Gene Symbol: Dectin-1 Gene ID (NCBI): 64581 RRID: AB_3085702 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXN2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.