Proteintech, 23153-1-AP, GOLPH3L Polyclonal antibody

Size: 20ul / 150ul The GOLPH3L (23153-1-AP) by Proteintech is a Polyclonal antibody targeting GOLPH3L in WB, IHC, ELISA applications with reactivity to human samples 23153-1-AP targets GOLPH3L in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, SK-BR-3 cells, T-47D cells Positive IHC detected in: human cervical cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information Golgi phosphoprotein 3 (GOLPH3) is a highly conserved membrane protein that plays an important role in maintaining the structure of the Golgi apparatus and anterograde transport. GOLPH3L(golgi phosphoprotein 3-like) is a GOLPH3 paralogue found in all vertebrate genomes. Compared with the oncogene GOLPH3, little is known about the role and function of GOLPH3L. It has been reported that GOLPH3L is highly expressed in epithelial ovarian cancer tissues, and cervical cancer tissue and is up-regulated in human rhabdomyosarcoma (PMID: 26284141; 28261346; 21822541). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19537 Product name: Recombinant human GOLPH3L protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 190-285 aa of BC013870 Sequence: VTNTTEKQRLVKKLQDSVLERWVNDPQRMDKRTLALLVLAHSSDVLENVFSSLTDDKYDVAMNRAKDLVELDPEVEGTKPSATEMIWAVLAAFNKS Predict reactive species Full Name: golgi phosphoprotein 3-like Calculated Molecular Weight: 285 aa, 33 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC013870 Gene Symbol: GOLPH3L Gene ID (NCBI): 55204 RRID: AB_3669408 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H4A5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.