Proteintech, 23306-1-AP, WWTR1/TAZ Polyclonal antibody

Size: 20ul / 150ul The WWTR1/TAZ (23306-1-AP) by Proteintech is a Polyclonal antibody targeting WWTR1/TAZ in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, rat samples 23306-1-AP targets WWTR1/TAZ in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, LN229 cells, MDA-MB-453s cells Positive IP detected in: MDA-MB-453s cells Positive IHC detected in: human ovary cancer tissue, human colon cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1. Specification Tested Reactivity: human, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19659 Product name: Recombinant human WWTR1,TAZ protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-400 aa of BC014052 Sequence: MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL Predict reactive species Full Name: WW domain containing transcription regulator 1 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC014052 Gene Symbol: WWTR1 Gene ID (NCBI): 25937 RRID: AB_2721185 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9GZV5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.