Proteintech, 23731-1-AP, TMEM231 Polyclonal antibody

Size: 20ul / 150ul The TMEM231 (23731-1-AP) by Proteintech is a Polyclonal antibody targeting TMEM231 in IHC, IF/ICC, ELISA applications with reactivity to human, canine samples 23731-1-AP targets TMEM231 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples. Tested Applications Positive IHC detected in: human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MDCK cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Transmembrane protein 231 (TMEM231) encodes a 36 kDa two-pass transmembrane protein that is part of the B9 complex and localizes to the base of the ciliary axoneme at the TZ. TMEM231 was first identified as a causative gene for MKS and participates in the formation of the diffusion barrier between the cilia and plasma membrane (PMID: 34912761, 37736303). Tmem231 mutant mice have developmental defects reminiscent of MKS patients, including corticomedullary kidney cysts, polydactyly and hepatic ductal plate malformation (PMID: 25869670). Specification Tested Reactivity: human, canine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20669 Product name: Recombinant human TMEM231 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 47-316 aa of BC010609 Sequence: GFWLKRSSYEEQPTVRFQHQVLLVALLGPESDGFLAWSTFPAFNRLQGDRLRVPLVSTREEDRNQDGKTDMLHFKLELPLQSTEHVLGVQLILTFSYRLHRMATLVMQSMAFLQSSFPVPGSQLYVNGDLRLQQKQPLSCGGLDARYNISVINGTSPFAYDYDLTHIVAAYQERNVTTVLNDPNPIWLVGRAADAPFVINAIIRYPVEVISYQPGFWEMVKFAWVQYVSILLIFLWVFERIKIFVFQNQVVTTIPVTVTPRGDLCKEHLS Predict reactive species Full Name: hypothetical protein FLJ22167 Calculated Molecular Weight: 345 aa, 39 kDa GenBank Accession Number: BC010609 Gene Symbol: TMEM231 Gene ID (NCBI): 79583 RRID: AB_2879312 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H6L2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.