Proteintech, 23955-1-AP, NSBP1 Polyclonal antibody

Size: 20ul / 150ul The NSBP1 (23955-1-AP) by Proteintech is a Polyclonal antibody targeting NSBP1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 23955-1-AP targets NSBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, HepG2 cells Positive IHC detected in: human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200 Background Information NSBP1, also named Nucleosome-binding protein 1 or HMGN5, is a 282 amino acid protein, which belongs to the HMGN family. NSBP1 localizes in the nucleus and is ubiquitously expressed. NSBP1 preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. Western blots shows the expression of NSBP1 as a 55kDa protein in human bladder cancer cells. (PMID: 21695596). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20884 Product name: Recombinant human NSBP1 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-282 aa of BC005342 Sequence: MPKRKAAGQGDMRQEPKRRSARLSAMLVPVTPEVKPKRTSSSRKMKTKSDMMEENIDTSAQAVAETKQEAVVEEDYNENAKNGEAKITEAPASEKEIVEVKEENIEDATEKGGEKKEAVAAEVKNEEEDQKEDEEDQNEEKGEAGKEDKDEKGEEDGKEDKNGNEKGEDAKEKEDGKKGEDGKGNGEDGKEKGEDEKEEEDRKETGDGKENEDGKEKGDKKEGKDVKVKEDEKEREDGKEDEGGNEEEAGKEKEDLKEEEEGKEEDEIKEDDGKKEEPQSIV Predict reactive species Full Name: nucleosomal binding protein 1 Calculated Molecular Weight: 282 aa, 32 kDa Observed Molecular Weight: 55 kDa GenBank Accession Number: BC005342 Gene Symbol: NSBP1 Gene ID (NCBI): 79366 RRID: AB_2879374 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: P82970 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.