Proteintech, 24054-1-AP, CNKSR2 Polyclonal antibody

Size: 20ul / 150ul The CNKSR2 (24054-1-AP) by Proteintech is a Polyclonal antibody targeting CNKSR2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 24054-1-AP targets CNKSR2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Positive IHC detected in: rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The Connector Enhancer of Kinase Suppressor of Ras-2 (CNKSR2), also known as CNK2 or MAGUIN, KIAA0902 and KSR2, is a scaffolding molecule that contains functional protein binding domains (PMID: 35053419). CNKSR2 interacts with different molecules, including RAF1, ARHGAP39, and CYTH2, and regulates the Mitogen-Activated Protein Kinase (MAPK) cascade and small GTPase signaling (PMID: 35053419). The study identify a molecular complex comprising CNKSR2 and the guanine nucleotide exchange factor (GEF) for ARF small GTPases, CYTH2, that is necessary for the proper development of granule neurons in the mouse hippocampus (PMID: 34800437). CNKSR2 plays a key role in regulating cell proliferation, and differentiation through multiple receptor tyrosine kinase pathways (PMID: 35053419). The molecular weight of CNKSR2 is mainly 117 kDa (PMID: 29534682). It has also been reported that CNKSR2 immunoblotted signal of about 110 kDa was detected, while a 130 kDa signal was detected (PMID: 34800437). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21183 Product name: Recombinant human CNKSR2 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 400-558 aa of BC126121 Sequence: RKRFNIVEEDTVLYCYEYEKGRSSSQGRRESTPTYGKLRPISMPVEYNWVGDYEDPNKMKRDSRRENSLLRYMSNEKIAQEEYMFQRNSKKDTGKKSKKKGDKSNSPTHYSLLPSLQMDALRQDIMGTPVPETTLYHTFQQSSLQHKSKKKNKGPIAGK Predict reactive species Full Name: connector enhancer of kinase suppressor of Ras 2 Calculated Molecular Weight: 1034 aa, 118 kDa Observed Molecular Weight: 110 kDa, 130 kDa GenBank Accession Number: BC126121 Gene Symbol: CNKSR2 Gene ID (NCBI): 22866 RRID: AB_3669430 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen Affinity purified UNIPROT ID: Q8WXI2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.