Proteintech, 24322-1-AP, ATG16L2 Polyclonal antibody

Size: 20ul / 150ul The ATG16L2 (24322-1-AP) by Proteintech is a Polyclonal antibody targeting ATG16L2 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 24322-1-AP targets ATG16L2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse spleen tissue, mouse testis tissue Positive IP detected in: mouse testis tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information ATG16L2 also named as Autophagy related protein 16-2 is a 619 amino-acid protein, Which belongs to the WD repeat ATG16 family. ATG16L2 contains 7 WD repeats and localizes in the cytoplasm. There are three isoforms of ATG16L2 and the molecular weight of three isoform is respectively 69kDa, 56kDa and 31kDa. ATG16L2 may play a role in autophagy. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19399 Product name: Recombinant human ATG16L2 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 17-365 aa of BC137490 Sequence: HIVRQLRLRDRTQKALFLELVPAYNHLLEKAELLDKFSKKLQPEPNSVTPTTHQGPWEESELDSDQVPSLVALRVKWQEEEEGLRLVCGEMAYQVVEKGAALGTLESELQQRQSRLAALEARVAQLREARAQQAQQVEEWRAQNAVQRAAYEALRAHVGLREAALRRLQEEARDLLERLVQRKARAAAERNLRNERRERAKQARVSQELKKAAKRTVSISEGPDTLGDGMRERRETLALAPEPEPLEKEACEKWKRPFRSASATSLTLSHCVDVVKGLLDFKKRRGHSIGGAPEQRYQIIPVCVAARLPTRAQDVLDAHLSEVNAVRFGPNSSLLATGGADRLIHLWNV Predict reactive species Full Name: ATG16 autophagy related 16-like 2 (S. cerevisiae) Calculated Molecular Weight: 619 aa, 69 kDa Observed Molecular Weight: 70 kDa, 30 kDa GenBank Accession Number: BC137490 Gene Symbol: ATG16L2 Gene ID (NCBI): 89849 RRID: AB_2879493 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8NAA4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.