Proteintech, 24333-1-AP, ANKRD6 Polyclonal antibody

Size: 20ul / 150ul The ANKRD6 (24333-1-AP) by Proteintech is a Polyclonal antibody targeting ANKRD6 in WB, IHC, IP, ELISA applications with reactivity to human samples 24333-1-AP targets ANKRD6 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, fetal human brain tissue Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse ovary tissue, human kidney tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Ankrd6 (Ankyrin repeat domain 6) is a distant homolog of the fly PCP mediator diego and acts as a molecular switch between both Wnt pathways. It promotes signaling of the non canonical Wnt/PCP pathway and simultaneously inhibits gene transcription through the canonical Wnt/β-catenin pathway. Specification Tested Reactivity: human Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19328 Product name: Recombinant human ANKRD6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-260 aa of BC042173 Sequence: MSQQDAVAALSERLLVAAYKGQTENVVQLINKGARVAVTKHGRTPLHLAANKGHLPVVQILLKAGCDLDVQDDGDQTALHRATVVGNTEIIAALIHEGCALDRQDKDGNTALHEASWHGFSQSAKLLVKAGANVLAKNKAGNTALHLACQNSHSQSTRVLLLAGSRADLKNNAGDTCLHVAARYNHLSIIRLLLTAFCSVHEKNQAGDTALHVAAALNHKKVAKILLEAGADTTIVNNAGQTPLETARYHNNPEVALLLT Predict reactive species Full Name: ankyrin repeat domain 6 Calculated Molecular Weight: 727 aa, 80 kDa Observed Molecular Weight: 80-90 kDa GenBank Accession Number: BC042173 Gene Symbol: ANKRD6 Gene ID (NCBI): 22881 RRID: AB_2879498 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2G4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.