Proteintech, 24439-1-AP, NUP205 Polyclonal antibody

Size: 20ul / 150ul The NUP205 (24439-1-AP) by Proteintech is a Polyclonal antibody targeting NUP205 in WB, IP, IHC, ELISA applications with reactivity to human samples 24439-1-AP targets NUP205 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information NUP205, also named as C7orf14 and KIAA0225, is a 205 kDa nucleoporin. It plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19832 Product name: Recombinant human NUP205 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1662-2012 aa of BC136624 Sequence: CQDVSAGSLQELALLTGIISKAALPGILSELDVDVNEGSLMELQGHIGRFQRQCLGLLSRFGGSDRLRQFKFQDDNVEGDKVSKKDEIELAMQQICANVMEYCQSLMLQSSPTFQHAVCLFTPSLSETVNRDGPRQDTQAPVVPYWRLPGLGIIIYLLKQSANDFFSYYDSHRQSVSKLQNVEQLPPDEIKELCQSVMPAGVDKISTAQKYVLARRRLVKVINNRAKLLSLCSFIIETCLFILWRHLEYYLLHCMPTDSQDSLFASRTLFKSRRLQDSFASETNLDFRSGLAIVSQHDLDQLQADAINAFGESLQKKLLDIEGLYSKVRSRYSFIQALVRRIRGLLRISRN Predict reactive species Full Name: nucleoporin 205kDa Calculated Molecular Weight: 2012 aa, 228 kDa Observed Molecular Weight: 205 kDa GenBank Accession Number: BC136624 Gene Symbol: NUP205 Gene ID (NCBI): 23165 RRID: AB_2879550 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92621 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.