Proteintech, 24446-1-AP, RCCD1 Polyclonal antibody

Size: 20ul / 150ul The RCCD1 (24446-1-AP) by Proteintech is a Polyclonal antibody targeting RCCD1 in WB, IHC, ELISA applications with reactivity to human samples 24446-1-AP targets RCCD1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells, Jurkat cells Positive IHC detected in: human liver cancer tissue, human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information RCCD1 is a new member of the large, phylogenetically conserved, RCC1 superfamily. Many RCC1 superfamily proteins have been linked to several human diseases, including cancer. RCCD1 was reported as a partner of histone H3K36 demethylase KDM8 involved in chromosome segregation, and has been identified as a potential driver for breast cancer. RCCD1 plays a role in regulating alpha-tubulin deacetylation and cytoskeletal microtubule stability, thereby promoting cell migration and TGF-beta-induced epithelial-to-mesenchymal transition (EMT), potentially through the inhibition of KDM8. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19685 Product name: Recombinant human RCCD1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-376 aa of BC140708 Sequence: MAEERPGAWFGFGFCGFGQELGSGRGRQVHSPSPLRAGVDICRVSASWSYTAFVTRGGRLELSGSASGAAGRCKDAWASEGLLAVLRAGPGPEALLQVWAAESALRGEPLWAQNVVPEAEGEDDPAGEAQAGRLPLLPCARAYVSPRAPFYRPLAPELRARQLELGAEHALLLDAAGQVFSWGGGRHGQLGHGTLEAELEPRLLEALQGLVMAEVAAGGWHSVCVSETGDIYIWGWNESGQLALPTRNLAEDGETVAREATELNEDGSQVKRTGGAEDGAPAPFIAVQPFPALLDLPMGSDAVKASCGSRHTAVVTRTGELYTWGWGKYGQLGHEDTTSLDRPRRVEYFVDKQLQVKAVTCGPWNTYVYAVEKGKS Predict reactive species Full Name: RCC1 domain containing 1 Calculated Molecular Weight: 376 aa, 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC140708 Gene Symbol: RCCD1 Gene ID (NCBI): 91433 RRID: AB_3085734 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: A6NED2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.