Proteintech, 24525-1-AP, DHX57 Polyclonal antibody

Size: 20ul / 150ul The DHX57 (24525-1-AP) by Proteintech is a Polyclonal antibody targeting DHX57 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 24525-1-AP targets DHX57 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 (DHX57), also named as DDX57, is a member of the DEAH subfamily within the Dead box protein family which is involved in modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This antibody reacts with 150 kDa DHX57 protein. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21743 Product name: Recombinant human DHX57 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 163-500 aa of BC131534 Sequence: PLEYAGLASVEPYVPEFTVSPFAVQKLSRYGFNTERCQAVLRMCDGDVGASLEHLLTQCFSETFGERMKISEAVNQISLDECMEQRQEEAFALKSICGEKFIERIQNRVWTIGLELEYLTSRFRKSKPKESTKNVQENSLEICKFYLKGNCKFGSKCRFKHEVPPNQIVGRIERSVDDSHLNAIEDASFLYELEIRFSKDHKYPYQAPLVAFYSTNENLPLACRLHISEFLYDKALTFAETSEPVVYSLITLLEEESEIVKLLTNTHHKYSDPPVNFLPVPSRTRINNPACHKTVIPNNSFVSNQIPEVEKASESEESDEDDGPAPVIVENESYVNLK Predict reactive species Full Name: DEAH (Asp-Glu-Ala-Asp/His) box polypeptide 57 Calculated Molecular Weight: 1386 aa, 156 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC131534 Gene Symbol: DHX57 Gene ID (NCBI): 90957 RRID: AB_2879590 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6P158 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.