Proteintech, 24722-1-AP, C14orf138 Polyclonal antibody

Size: 20ul / 150ul The C14orf138 (24722-1-AP) by Proteintech is a Polyclonal antibody targeting C14orf138 in WB, IHC, ELISA applications with reactivity to human, mouse samples 24722-1-AP targets C14orf138 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: PC-3 cells, HepG2 cells, human testis tissue Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:200-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information C14orf138, also named as VCPKMT or METTL21D, is 229 amino acid protein, which belongs to the methyltransferase superfamily. C14orf138 localizes in the cytoplasm and interacts with ALKBH6. C14orf138 as a Protein-lysine N-methyltransferase that specifically trimethylates 'Lys-315' of VCP/p97; this modification may decrease VCP ATPase activity. C14orf138 exists as five isoforms. The calculated molecular weight of C14orf138 is 26 kDa,but we always detected a 46-50 kDa portein by western blot. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20154 Product name: Recombinant human C14orf138 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-144 aa of BC105118 Sequence: MADTLESSLEDPLRSFVRVLEKRDGTVLRLQQYSSGGVGCVVWDAAIVLSKYLETPEFSGDGAHALSRRSVLELGSGTGAVGLMAATLGADVVVTDLEELQDLLKMNINMNKHLVTGSVQAKGGRNRRLSFSTRLHTDGRLHIL Predict reactive species Full Name: chromosome 14 open reading frame 138 Calculated Molecular Weight: 229 aa, 26 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC105118 Gene Symbol: C14orf138 Gene ID (NCBI): 79609 RRID: AB_2879689 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H867 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.