Proteintech, 24759-1-AP, ZDHHC13 Polyclonal antibody

Size: 20ul / 150ul The ZDHHC13 (24759-1-AP) by Proteintech is a Polyclonal antibody targeting ZDHHC13 in WB, IHC, ELISA applications with reactivity to human, mouse samples 24759-1-AP targets ZDHHC13 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, HEK-293 cells, mouse skin tissue, mouse testis tissue, Neuro-2a cells Positive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Palmitoyltransferase ZDHHC13 also named as HIP14L or DHHC13 is a 622 amino acid protein, which contains one DHHC-type zinc finger and six ANK repeats. ZDHHC13 localizes in the Golgi apparatus membrane and belongs to the DHHC palmitoyltransferase family. ZDHHC13 is a palmtoyltransferase for HD and GAD2, meanwhile it mediates Mg2+ transportation. ZDHHC13 exists as three alternative splicing isoforms. The molecular weight of isoform 2 is 51 kDa, but we often detected a 45-50kDa protein by western blot. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21607 Product name: Recombinant human ZDHHC13 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 529-622 aa of BC056152 Sequence: FHFSWSTFLLLNQLFQIAFLGLTSHERISLQKQSKHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCVVDWTSQYTMVFHPAREKVLRSV Predict reactive species Full Name: zinc finger, DHHC-type containing 13 Calculated Molecular Weight: 622 aa, 71 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC056152 Gene Symbol: ZDHHC13 Gene ID (NCBI): 54503 RRID: AB_2879708 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IUH4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.