Proteintech, 24761-1-AP, PTGER3 Polyclonal antibody

Size: 20ul / 150ul The PTGER3 (24761-1-AP) by Proteintech is a Polyclonal antibody targeting PTGER3 in WB, IHC, ELISA applications with reactivity to human, mouse samples 24761-1-AP targets PTGER3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse brain tissue, mouse cerebellum tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information PTGER3 (Prostaglandin E Receptor 3), also known as EP3, is a G protein-coupled receptor (GPCR). Activates pathways involved in proliferation, migration, and apoptosis, often via cross-talk with PI3K-AKT and MAPK cascades (PMID: 38566528). High PTGER3 levels drive tumorigenesis, angiogenesis, and cisplatin resistance via the Ras-MAPK/Erk-ETS1-ELK1/CFTR1 axis (PMID: 30655206). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag21542 Product name: Recombinant human PTGER3 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-100 aa of BC024229 Sequence: MKETRGYGGDAPFCTRLNHSYTGMWAPERSAEARGNLTRPPGSGEDCGSVSVAFPITMLLTGFVGNALAMLLVSRSYRRRESKRKKSFLLCIGWLALTDL Predict reactive species Full Name: prostaglandin E receptor 3 (subtype EP3) Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40-48 kDa GenBank Accession Number: BC024229 Gene Symbol: PTGER3 Gene ID (NCBI): 5733 RRID: AB_2879710 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P43115 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.