Proteintech, 24790-1-AP, GIGYF2 Polyclonal antibody

Size: 20ul / 150ul The GIGYF2 (24790-1-AP) by Proteintech is a Polyclonal antibody targeting GIGYF2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 24790-1-AP targets GIGYF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, NIH/3T3 cells Positive IP detected in: Jurkat cells Positive IHC detected in: mouse brain tissue, rat brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information GIGYF2 (GRB10 interacting GYF protein 2) was initially identified through its interaction with GRB10, an adapter protein that binds activated INS-like growth factor (IGF-I) and INS receptors (PMID: 12771153; 19744960). It may act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. The gene of GIGYF2 has been proposed as the Parkinson-disease (PD) gene underlying the PARK11 locus (PMID: 18358451). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20329 Product name: Recombinant human GIGYF2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 777-1104 aa of BC146775 Sequence: KRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELEVQRQKELMRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPSGTTKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSNADTKNSNMGFWDDAV Predict reactive species Full Name: GRB10 interacting GYF protein 2 Calculated Molecular Weight: 1320 aa, 152 kDa Observed Molecular Weight: 150-170 kDa GenBank Accession Number: BC146775 Gene Symbol: GIGYF2 Gene ID (NCBI): 26058 RRID: AB_2879727 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6Y7W6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.