Proteintech, 24849-1-AP, VISTA Polyclonal antibody

Size: 20ul / 150ul The VISTA (24849-1-AP) by Proteintech is a Polyclonal antibody targeting VISTA in WB, IHC, IF-P, ELISA applications with reactivity to human samples 24849-1-AP targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue, SH-SY5Y cells, human peripheral blood platelets Positive IHC detected in: human placenta tissue, human colon cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human placenta tissue, human tonsillitis tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H and SISP1, is a member of the immunoglobulin superfamily. It serves as not only as a positive regulator but also as a substrate of MT1-MMP, and contributes at least in part to tumor invasion. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20600 Product name: Recombinant human C10orf54 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 34-196 aa of BC020568 Sequence: KVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAAAL Predict reactive species Full Name: chromosome 10 open reading frame 54 Calculated Molecular Weight: 311 aa, 34 kDa Observed Molecular Weight: 45-60 kDa GenBank Accession Number: BC020568 Gene Symbol: VISTA Gene ID (NCBI): 64115 RRID: AB_2879755 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9H7M9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.