Proteintech, 24891-1-AP, EVI2B Polyclonal antibody

Size: 20ul / 150ul The EVI2B (24891-1-AP) by Proteintech is a Polyclonal antibody targeting EVI2B in WB, IHC, ELISA applications with reactivity to human samples 24891-1-AP targets EVI2B in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, A549 cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:800-1:3200 Background Information Ecotropic viral integration site 2B protein (EVI2B) is a type I transmembrane protein embedded within intron 27b of the neurofibromatosis type 1 (NF1) gene. It is transcribed in the opposite direction to the NF1 gene (PMID: 37584733). EVI2B is a downstream target of CCAAT/enhancer binding protein alpha (C/EBPα), which is crucial for myeloid differentiation and the function of hematopoietic progenitors (PMID: 36830696). This protein is highly expressed in mature granulocytes and is involved in the regulation of immune cell maturation (PMID: 28186500). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag19511 Product name: Recombinant human EVI2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 236-448 aa of BC005926 Sequence: QNWAGRSPFADGETPDICMDNIRENEISTKRTSIISLTPWKPSKSTLLADDLEIKLFESSENIEDSNNPKTEKIKDQVNGTSEDSADGSTVGTAVSSSDDAGLPPPPPLLDLEGQESNQSDKPTMTIVSPLPNDSTSLPPSLDCLNQDCGDHKSEIIQSFPPLDSLNLPLPPVDFMKNQEDSNLEIQCQEFSIPPNSDQDLNESLPPPPAELL Predict reactive species Full Name: ecotropic viral integration site 2B Calculated Molecular Weight: 448 aa, 49 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC005926 Gene Symbol: EVI2B Gene ID (NCBI): 2124 RRID: AB_2879781 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P34910 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.