Proteintech, 24933-1-AP, KEPI Polyclonal antibody

Size: 20ul / 150ul The KEPI (24933-1-AP) by Proteintech is a Polyclonal antibody targeting KEPI in WB, ELISA applications with reactivity to human samples 24933-1-AP targets KEPI in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information PPP1R14C, found as a morphine‐regulated brain gene, encodes protein phosphatase 1 regulatory inhibitor subunit 14C, and is identified as a potent inhibitor of PP1. Horvath and colleagues reported that PPP1R14C inhibited the release of neurotransmitters and promoted neuronal exocytosis by retaining synaptosome associated protein 25 (SNAP25) at a phosphorylated state. Moreover, overexpression of PPP1R14C increased the phosphorylation of RB transcriptional co‐repressor 1 (RB1) to protect leukemic cells from chemotherapy. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag20645 Product name: Recombinant human KEPI protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-165 aa of BC015773 Sequence: MSVATGSSEAAGGASGGGARVFFQSPRGGAGGSPGSSSGSGSSREDSAPVATAAAAGQVQQQQQRRHQQGKVTVKYDRKELRKRLVLEEWIVEQLGQLYGCEEEEMPEVEIDIDDLLDADSDEERASKLQEALVDCYKPTEEFIKELLSRIRGMRKLSPPQKKSV Predict reactive species Full Name: protein phosphatase 1, regulatory (inhibitor) subunit 14C Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 20-22 kDa GenBank Accession Number: BC015773 Gene Symbol: PPP1R14C Gene ID (NCBI): 81706 RRID: AB_3085762 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TAE6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.