Proteintech, 25167-1-AP, ALDH1A3 Polyclonal antibody

Size: 20ul / 150ul The ALDH1A3 (25167-1-AP) by Proteintech is a Polyclonal antibody targeting ALDH1A3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 25167-1-AP targets ALDH1A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue, rat liver tissue Positive IHC detected in: human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Aldehyde dehydrogenase 1 family member A3 (ALDH1A3), is an aldehyde dehydrogenase enzyme that uses retinal as a substrate either in a free or a cellular retinol-binding protein form. ALDH1A3 plays a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation.ALDH1A3 is calculated to be 56 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, goat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18605 Product name: Recombinant human ALDH1A3 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-163 aa of BC069274 Sequence: MATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVV Predict reactive species Full Name: aldehyde dehydrogenase 1 family, member A3 Calculated Molecular Weight: 512 aa, 56 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC069274 Gene Symbol: ALDH1A3 Gene ID (NCBI): 220 RRID: AB_2879937 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P47895 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.