Proteintech, 25233-1-AP, MARF1 Polyclonal antibody

Size: 20ul / 150ul The MARF1 (25233-1-AP) by Proteintech is a Polyclonal antibody targeting MARF1 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 25233-1-AP targets MARF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human small intestine tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information MARF1, also known as KIAA0430, or LKAP, which is expressed in peroxisome. MARF1 is essential regulator of oogenesis and required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. MARF1 probably acts via some RNA metabolic process and is equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18905 Product name: Recombinant human KIAA0430 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-286 aa of BC137165 Sequence: MMEGNGTENSCSRTRGWLQQDNDAKPWLWKFSNCFSRPEQTLPHSPQTKEYMENKKVAVELKDVPSPLHAGSKLFPAVPLPDIRSLQQPKIQLSSVPKVSCCAHCPNEPSTSPMRFGGGGGGSGGTSSLIHPGALLDSQSTRTITCQVGSGFAFQSASSLQNASARNNLAGIASDFPSMCLESNLSSCKHLPCCGKLHFQSCHGNVHKLHQFPSLQGCTSAGYFPCSDFTSGAPGHLEEHISQSELTPHLCTNSLHLNVVPPVCLKGSLYCEDCLNKPARNSIIDA Predict reactive species Full Name: KIAA0430 Calculated Molecular Weight: 1742 aa, 193 kDa Observed Molecular Weight: 192 kDa GenBank Accession Number: BC137165 Gene Symbol: MARF1 Gene ID (NCBI): 9665 RRID: AB_2879977 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y4F3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.