Proteintech, 25268-1-AP, ORP1L Polyclonal antibody

Size: 20ul / 150ul The ORP1L (25268-1-AP) by Proteintech is a Polyclonal antibody targeting ORP1L in WB, ELISA applications with reactivity to human, mouse, rat samples 25268-1-AP targets ORP1L in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse brain tissue, rat brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:3000 Background Information ORP1L (Oxysterol-binding protein-related protein 1L) is a lipid transfer protein that belongs to the family of OSBP-related proteins (ORPs). It is recruited to lysosomes via its ankyrin repeat domains that bind to Rab7(PMID: 34504082). ORP1L also possesses an FFAT motif, which allows it to interact with the ER resident VAP proteins to aid in establishing the ER-lysosomes contacts. ORP1L is expressed in various tissues, with particularly high expression in the brain, lungs, and macrophages. ORP1L plays a crucial role in cholesterol homeostasis. Disruption of ORP1L function can lead to a block in cholesterol transport to the ER, resulting in the accumulation of cholesterol-enriched LELs in the Golgi/perinuclear region. This can disrupt cellular cholesterol balance, potentially contributing to various metabolic diseases. This suggests that ORP1L is necessary for the normal functioning of cholesterol export from the endo-lysosomal system to the ER, and its dysfunction could be implicated in the progression of diseases related to cholesterol metabolism dysregulation(PMID: 28564600). The molecular weight of ORP1L is 50 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: oxysterol binding protein-like 1A Calculated Molecular Weight: 108 kDa Observed Molecular Weight: 50 kDa, 105-110 kDa GenBank Accession Number: NM_018030 Gene Symbol: OSBPL1A Gene ID (NCBI): 114876 RRID: AB_3669468 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BXW6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.