Proteintech, 25293-1-AP, CWC15 Polyclonal antibody

Size: 20ul / 150ul The CWC15 (25293-1-AP) by Proteintech is a Polyclonal antibody targeting CWC15 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 25293-1-AP targets CWC15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: mouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells Positive IP detected in: HeLa cells Positive IHC detected in: human stomach tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag18116 Product name: Recombinant human CWC15 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC040946 Sequence: MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK Predict reactive species Full Name: CWC15 spliceosome-associated protein homolog (S. cerevisiae) Calculated Molecular Weight: 229 aa, 27 kDa Observed Molecular Weight: 27-35 kDa GenBank Accession Number: BC040946 Gene Symbol: CWC15 Gene ID (NCBI): 51503 RRID: AB_2880012 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9P013 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.