Proteintech, 25472-1-AP, MXRA5 Polyclonal antibody

Size: 20ul / 150ul The MXRA5 (25472-1-AP) by Proteintech is a Polyclonal antibody targeting MXRA5 in WB, IHC, ELISA applications with reactivity to human samples 25472-1-AP targets MXRA5 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells Positive IHC detected in: human colon cancer tissue, human kidney tissue, human liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MXRA5, or adlican, is an adhesion proteoglycan involved in extracellular matrix (ECM) remodeling and cell-cell adhesion (PMID: 22696596). In kidney, MXRA5 has anti-inflammatory and anti-fibrotic properties by limiting the induction of chemokines, fibronectin and collagen expression in response to TGB1 and pro-inflammatory stimuli (PMID: 27599751). MXRA5 was identified as a novel biomarker in colorectal cancer, mainly located in the cytoplasm of CRC cells (PMID:23420087). Exome sequencing identified MXRA5 as a novel cancer gene mutated in non-small cell lung carcinoma (PMID: 22696596). MXRA5 was detected 163 kDa in human proximal tubular cells (PMID:27599751). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: matrix-remodelling associated 5 Calculated Molecular Weight: 312 kDa Observed Molecular Weight: 163 kDa, 312 kDa GenBank Accession Number: NM_015419 Gene Symbol: MXRA5 Gene ID (NCBI): 25878 RRID: AB_2918088 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NR99 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.